![]() ![]() o Record the pH value, corresponding to the color change, in the patient’s chart. o The paper may be applied directly to the pooled vaginal fluid. DO NOT allow it to come into contact with any liquid or other substance, which might affect pH. The amniotic liquid surrounding the fetus is quite essential to the baby's development. SPECIMEN AND TESTING PROCEDURE: o Remove 1-2 inches of pH paper from the holder for each test. Amniotic fluid is a liquid of a clear or a little yellow color that is found in the uterus of pregnant women, which can becalled liquor amnii. Do not use pH paper from containers without a QC label. Amniotic fluid also helps develop your baby’s digestive and respiratory system, as well as regulates their temperature. Amniotic fluid is a clear, yellow fluid which is found within the first 12 days following conception within the amniotic sac. It protects your baby from infection and umbilical cord compression and cushions their movements while they’re in your uterus. Labels will be attached to the pH dispensers indicating that the quality control has been performed. Amniotic fluid is a water-like fluid that surrounds your baby in your uterus. ![]() ![]() New orders will be QC’d prior to distribution to sites. QUALITY CONTROL: The laboratory will complete all Quality Control. REAGENTS, EQUIPMENT AND MATERIALS: o pH paper (Hydrion 3 to 9 paper) o pH Color Card o Sterile Gloves STORAGE: pH paper must be stored in its container at room temperature. The pH of the upper vagina is normally acidic, while amniotic fluid has a neutral pH. At birth, meconium can be seen in the amniotic fluid. The colors correlate to a pH range of 3 to 9 and indicate the pH of the specimen. Dark, greenish staining or streaking of the amniotic fluid or the obvious presence of meconium in the amniotic fluid Limpness in infant at birth Diagnosis Before birth, the fetal monitor may show a slow heart rate. In the presence of acidic fluids, the paper will range from red-orange to orange in color and in the presence of alkaline fluids, the paper will range from green to blue in color. In the presence of fluid with normal pH (7.0), the paper will turn a yellow-green color. Either sign requires immediate medical attention. It could also indicate meconium, which is what is in the babys digestion system. If you have liquid coming out that is more green in color, that could point to an infection, particularly if it has a bad smell. PRINCIPLE: pH paper is a test strip used for the determination of pH. Call your doctor if the fluid is green, brown, or smells bad. The presence of amniotic fluid tends to elevate the pH, and detection of a pH increase using pH paper may assist in determining the presence of amniotic fluid. Rupture of the amniotic membrane can result in leakage of small volumes of amniotic fluid in the upper vagina. Vaginal/Amniotic Fluid: pH determination is used as a screening system for detecting the rupture of the amniotic membrane in pregnant women. PH Testing in Amniotic Fluid MCLNO-Pathology Service Waived Testing Unit PURPOSE: To determine pH in vaginal pool. ![]()
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